635-nm 발광 다이오드가 임플란트 주위염 in vitro 모형에 미치는 효과
The Effects of 635-nm Light-Emitting Diode Irradiation in Peri-Implantitis in vitro Model
조민성, 최창우, Karna Sandeep, 임원봉, 김지선, 김상우, Hui Zeng, 전상미, 오희균, 김옥준, 최홍란,
소속 상세정보
조민성 ( Cho Min-Sung ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
최창우 ( Choi Chang-Woo ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
( Karna Sandeep ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
임원봉 ( Lim Won-Bong ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김지선 ( Kim Ji-Sun ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
김상우 ( Kim Sang-Woo ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
( Hui Zeng ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
전상미 ( Jeon Sang-Mi ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
오희균 ( Oh Hee-Kyun ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
김옥준 ( Kim Ok-Joon ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
최홍란 ( Choi Hong-Ran ) - 전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소
KMID : 0829220120360040219
Abstract
Peri-implantitis (PI) is bacteria-induced inflammatory condition which affects the alveolar bone and soft tissue around implants and may result in the loss of supporting bone. Attenuation of the P. gingivalis lipopolysaccharide (LPS)-induced inflammatory response can be a new therapeutic approach in the treatment of PI. This study was conducted to evaluate the anti-inflammatory effect of 635-nm light-emitting diode (LED) irradiation over MG63 osteoblast-like cell. Scratch was made on MG63 cells with or without LPS, then 635-nm irradiated. The expression of the cyclooxygenase-2 (COX-2) proteins was evaluated with western blot. The production of the prostaglandin E2 (PGE2) and expression of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) was measured with enzyme-linked immunoassay, and the cytokine profile was evaluated with the human inflammation antibody array. Wound closure effect presented in the cells treated with LPS was observed more significantly in the cells with 635-nm irradiation than the cells without irradiation. The 635-nm irradiaiton reduced LPS-induced expression of the COX-2 and production of the PGE2. Also, 635-nm irradiation affect the expression of RANKL, OPG, and proinflammatory cytokines. These results indicate that 635-nm irradiation could reduce the alveolar bone resorption induced by LPS stimulation through the inhibition of COX-2 expression and PGE2 production, the suppression of proinflammatory cytokine, and the modulation of RANKL/OPG balance in MG63 cells.
키워드
LED; Pperi-implantitis
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